Human Nail Clippings as a Source of DNA for Genetic Studies

• 作者: Argyrios Ziogas Hannah Lui Park Hoda Anton-Culver Le Truong Leslie Bernstein Seong Sil Chang Sophia S. Wang Susan L. Neuhausen
• 来源期刊: 流行病学期刊(英文)
• 学科: 医学
• 关键词: Single NUCLEOTIDE Polymorphism (SNP) NAIL Clippings GENOTYPING WHOLE Genome AMPLIFICATION (WGA)
• 年，卷（期）: 2015,（1）
• 页码范围: 41-50
• 页数: 10页
• 分类号: R73

摘要

Blood samples have traditionally been used as the main source of DNA for genetic analysis. How-ever, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we investigated whether human nail clippings could be used as a source of DNA for SNP genotyping, null-allele detection, and whole-genome amplification. From extracted nail DNA, we achieved amplicons up to a length of ~400 bp and >96% concordance for SNP genotyping and 100% concordance for null-allele detection compared to DNA derived from matched blood sam-ples. For whole-genome amplification, OmniPlex performed better than Multiple Displacement Amplification with a success rate of 89.3% and 76.8% for SNP genotyping and null-allele detection, respectively. Concordance was ~98% for both methods. When combined with OmniPlex whole-genome amplification, human nail clippings could potentially be used as an alternative to whole blood as a less invasive and more convenient source of DNA for genotyping studies.