Construction of cDNA Library from the Antenna of Chrysopa pallens（ Rambur）

• 作者: Chen Hongyin Liu Chenxi Wang Juan Wang Mengqing Zhang Haiping Zhang Lisheng
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 生物学
• 关键词: 全长CDNA文库 淡色库蚊 文库构建 天线 草蛉 相关基因 DNA连接 切换机制
• 年，卷（期）: 2015,（3）
• 页码范围: 14-19
• 页数: 6页
• 分类号: Q785

摘要

A full-length c DNA library from the antenna of Chrysopa pallens( Rambur) was constructed based on switching mechanism at 5’ end of RNA transcript( SMART) system. The purified double-stranded c DNA was ligated to vector SMART2 IFD,and transformed into Escherichia coli DH5ɑ by electroporation. The library constructed for female and male reached high titers of 2. 1 × 10~6 and 1. 8 × 10~6 pfu / m L,respectively. PCR results showed that the inserts varied from 400 bp to 2 000 bp with the average size larger than 500 bp and the recombination rate over 93. 0%. Further studies on those genes and large scale sequencing of the library may be helpful in screening new olfactory related genes in C. pallens.