Spectrophotometric Data in Human Immunodeficiency Virus (HIV)-Antiretroviral Drug Coated Blood Interactions

• 作者: Adaeze Ani Jeremiah Chukwuneke Okwuchukwu Ani
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: ABSORBANCE Human IMMUNODEFICIENCY Virus ANTIRETROVIRAL Drug BLOOD Components WAVELENGTH
• 年，卷（期）: 2015,（8）
• 页码范围: 44-52
• 页数: 9页
• 分类号: R5

摘要

The spectrophotometric data in the interactions between the Human immunodeficiency virus (HIV) and blood cells treated with antiretroviral drug were collected to be used to show the effects of antiretroviral drugs on the absorbance characteristics of HIV infected and uninfected blood. The methodology involved the serial dilution of the five different antiretroviral drugs (two HAART/FDC and three single drugs) and the subsequent incubation with the blood samples collected from ten HIV infected persons who had not yet commenced treatment with the antiretroviral drugs, ten HIV infected persons who had already commenced treatment with the antiretroviral drugs, and ten HIV negative persons, for the absorbance measurements using a digital Ultraviolet Visible MetaSpecAE1405031Pro Spectrophotometer. The peak absorbance data for various interacting systems were measured. These were used to show that the antiretroviral drug had the effect of increasing the peak absorbance values of both the uninfected and infected blood components, i.e., the drugs were made able to increase the light absorption capacity of the blood cells. The use of the findings of this work in drug design may be expected to yield good results.