This report is aimed to present the result of experimental setup of the erbium-doped fiber amplifier (EDFA) with modulated pulse signal by laser pumping at wavelength 980 nm. The amplified spontaneous emission (ASE) from EDFA has multi wavelength and the spacing of wavelength can be controlled by controlling the pulse width of laser pumping. The result in this experiment shows that the feasibility of using EDFA system can generate the multi wavelength of all of C-Band spectrum. The pulse signal, for modulated laser pumping, is observed ranging from 10 to 100 Hz and the wavelength spacing can be tuned from 14.7 nm to 14.9 nm.