One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2015,（2）
• 页码范围: 258-261
• 页数: 4页
• 语种: 中文
• DOI: 10.1038/cr.2014.158

摘要

Dear Editor, Non-human primates (NHPs) are genetically,physiologically and psychologically more similar to humans than other model organisms,providing an important model for human development and diseases [1,2].Precise genome modification in non-human primates (NHPs) holds great promise for biomedical researches.However,owing to the long sexual maturation time and low reproduction rate of NHPs,it is very challenging to generate biallelic mutant NHPs for loss-of-function studies through breeding.