Isoflavone and Mineral Content in Conventional and Transgenic Soybean Cultivars

• 作者: Gabriela Rezende Costa José Marcos Gontijo Mandarino Nilson César Castanheira Guimar?es Nilton de Oliveira Couto e Silva Renata Adriana Labanca Roberto Gon?alves Junqueira Rodrigo Santos Leite
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: GLYCINE max Genetically Modified ORGANISM Bioactive Compounds ICP-OES HPLC
• 年，卷（期）: 2015,（13）
• 页码范围: 2051-2059
• 页数: 9页
• 分类号: R73

摘要

The objective of this study was to evaluate the differences in composition among six brands of conventional soybean and six genetically modified cultivars (GM). We focused on the isoflavones profile and mineral content questioning the substantial equivalence between conventional and GM organisms. The statement of compliance label for conventional grains was verified for the presence of genetic modified genes by real time polymerase chain reaction (PCR). We did not detect the presence of the 35S promoter in commercial samples, indicating the absence of transgene insertion. For mineral analysis, we used the method of inductively coupled plasma-optical emission spectrometry (ICP-OES). Isoflavones quantification was performed by high performance liquid chromatography (HPLC). The results showed no statistical difference between the conventional and transgenic soybean groups concerning isoflavone content and mineral composition. The concentration of potassium, the main mineral component of soy, was the highest in conventional soybeans compared to that in GM soy, while GM samples presented the highest concentrations of iron.