Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis

• 作者: Christiane Maria de Oliveira Danúbia Aparecida de Carvalho Selvati Rezende David Lee Nelson Guilherme álvaro Ferreira da Silva Juliana de Andrade Santiago Luana Isac Soares Marcos Schleiden Sousa Carvalho Maria das Gra?as Cardoso Maria Luisa Teixeira Marisa Ionta Marisi Gomes Soares Rafaela Vieira de Souza
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: Cancer VOLATILE OIL Escova-de-Garrafa (Bottle Brush)
• 年，卷（期）: mgzwxqkyw_2015,（16）
• 页码范围: 2664-2671
• 页数: 8页
• 分类号: R73

摘要

The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.