Taking natural grassland on the northern slope of the Qilian Mountain for example, this paper investigated and compared aboveground and belowground biomass of grassland in multi-year enclosure(20 years), one-year enclosure, control areas(natural grazing areas). The results showed that coverage and height of the enclosure sample plots were significantly higher than that of natural grazing area(P <0.05); mean aboveground biomass of grassland: multi-year enclosure(316.58 g/m~2) > one-year enclosure area(299.07 g/m~2) > multi-year enclosure control area(254.39 g/m~2) > one-year enclosure control area(187.37 g/m~2); belowground biomass: multi-year enclosure(2,906.90 g/m~2) > one-year enclosure area(2,587.26 g/m~2) > multi-year enclosure control area(2,378.93 g/m~2) > one-year enclosure control area(2,029.17 g/m~2); mean aboveground biomass of natural grassland was 263.60 g/m~2, mean belowground biomass 2,225.56 g/m~2; ratio of belowground biomass to aboveground biomass varied between 6.79 and 12.90, distribution of belowground biomass and aboveground biomass in each plot showed significant differences(P <0.05). Enclosure was favorable for improving the coverage and biomass of natural grassland plant communities in the Qilian Mountains.