Amino acids essential for the interaction between cellular heat shock protein 90 and a Kaposi's sarcoma-associated herpesvirus-encoded protein kinase ORF36

• 来源期刊: 中国病毒学
• 年，卷（期）: 2016,（6）
• 页码范围: 509-512
• 页数: 4页
• 语种: 中文
• DOI: 10.1007/s12250-016-3839-9

摘要

Dear Editor, Protein-protein interactions (PPIs) often play important roles in biological processes (Zhang et al.,2016).The split Renilla luciferase complementation assay (SRLCA) is one of the methods in studying PPIs.SRLCA is based on the complementation of the N-terminal domains of Renilla luciferase (LN) and C-terminal domains of Renilla luciferase (LC) non-functional halves of Renilla luciferase fused to possibly interacting proteins and emit luminescence (Deng et al.,2011;Jiang et al.,2010)(Supplementary Figure S 1A).