Community Structure of Endophytic Bacteria in Chestnut, Castanea mollissima

• 作者: Chen Zebin Li Bing Liu Jiani Wang Dingkang Xia Tiyuan Xu Shengguang Yu Lei Zhang Yongfu
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 年，卷（期）: 2016,（4）
• 页码范围: 1-5
• 页数: 5页
• 分类号: S1

摘要

[Objective]The paper was to detect the species of endophytic bacteria in Chestnut (Castanea mollissima).[Method]The 16S rDNA-V4 region of endophytic bacteria in chestnut(sample name BL) was sequenced by Illumina Mi Seq high-throughput sequencing technology. The number of sequences and operational taxonomic units(OTUs) for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed.[Result]The number of effective sequences and OTUs were 21 167 /191. The rarefaction curves showed that adequate sampling was achieved,and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera,including Koribacter(3. 48%),Solibacter(2. 59%),Bradyrhizobium(3. 57%), Rhodoplanes(7. 41%), Methylobacterium(4. 20%), Agrobacterium(19. 73%), Kaistobacter( 2. 05%), Sphingomonas(18. 66%),Acidovorax(35. 98%),and Methylibium(2. 32%). The dominant species were Acidovorax,Agrobacterium and Sphingomonas.[Conclusion]Illumina high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria in chestnut.