[Objective]The content of total antimony(Sb) in fresh fruit was investigated and its pollution was evaluated,to provide the basic data for diet exposure evaluation.[Method]The content of total Sb in 194 samples was detected by hydride generation-atomic fluorescence spectrometry(HG-AFS),and the Sb pollution in several fruits was evaluated by the method of single factor pollution index. [Result]The mean of total Sb was 0.005 6 mg / kg,and the median was 0.003 8mg / kg. The minimum value was not detected while the maximum value was 0.21 mg / kg,and the confidence interval was ND-0.019 mg / kg(P5-P95). The medians of total Sb content in different fruits successively were dragon fruit(0.011 0 mg / kg) > kiwi fruit(0.008 0 mg / kg) > peach(0.006 0 mg / kg). According to the method of single factor pollution index,the index from all samples was less than 0.2,suggesting that the samples involved were under the normal background value.[Conclusion]The Sb was detected in all samples but under the background value,and it was concluded that there was no Sb pollution in fresh fruit in Hunan Province.