Isolation of Antagonistic Bacteria against graminearum and Its Antagonistic Activity

• 作者: Huang Shi Li Hongyan Ma Dongfang Sun Cai Zhang Changqing Zhang Peng
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: 小麦赤霉病菌 拮抗细菌 抑菌活性 分离 RDNA序列分析 拮抗作用 生化鉴定 植物病原真菌
• 年，卷（期）: pdapzwbchyj_2016,（5）
• 页码范围: 13-15
• 页数: 3页
• 分类号: S435.121.4

摘要

[Objective] To develop biological control resources of endophytic bacteria in wheat,the bacteria with antagonistic effect against Fusarium graminearum were screened and their antagonistic activity was studied. [Method] The endophytic bacteria of wheat with antagonistic effect were screened using plate confrontation method; the antagonistic bacteria were identified through physiological and biochemical characteristics and 16 S r DNA sequence analysis; the antagonistic spectrum of antagonistic bacteria was studied using plate confrontation method. [Result]The antagonistic bacterium XY-3 was isolated from wheat leaves,and was identified as Bacillus amyloliquefaciens according to the physiological and biochemical characteristics and 16 S r DNA sequence analysis. [Conclusion] XY-3 had antagonistic effect against four kinds of common pathogenic fungi,presenting broad spectrum antagonistic activity against plant pathogenic fungi.