4-35 Identification of Mutant Gene Induced by Carbon Ion Beams in Arabidopsis by Whole Genome Re-sequencing and Rough Map-based Cloning

• 作者: Chen Xia Chen Yuze Cui Tao Du Yan Feng Hui Li Wenjian Luo Shanwei Mu Jinhu Yu Lixia Zhou Libin
• 来源期刊: 中国科学院近代物理研究所和兰州重离子研究装置年报：英文版
• 学科: 物理学
• 年，卷（期）: 2016,（1）
• 页码范围: 153-154
• 页数: 2页
• 分类号: O4

摘要

In recent years,heavy ion beams have been recognized as an effective and efficient physical mutagen due to their capacity to induce mutations with high frequency and broad spectrum.Nowadays,it is not so difficult to produce various mutants in plants.However,to mine the responsible mutated genes has been an important challenge.For mutation isolation,map-based cloning is one of the major traditional ways to isolate the mutant genes that control traits of interest in forward genetics studies.However,the process of map-based cloning is usually complicated and time-consuming.