4-52 Control of Alternative Splicing of Caspase-9 Enhances the Radiosensitivity of Cancer Cells to Ionizing Radiations

• 作者: Jin Xiaodong Kuang Yanbei Li Ping Li Qiang Liu Xiongxiong
• 来源期刊: 中国科学院近代物理研究所和兰州重离子研究装置年报：英文版
• 学科: 物理学
• 年，卷（期）: 2016,（1）
• 页码范围: 177-177
• 页数: 1页
• 分类号: O4

摘要

The splicing of caspase-9 gene generates two isoforms such as caspase-9a and caspase-9b/9S,which can be generated by the inclusion or exclusion of a 4-exon cassette including exon 3,4,5,and 6 in the mature caspase-9 mRNA[1].To investigate whether artificial increase of caspase-9a/9b mRNA ratio could enhance the sensitivity of non-small cell lung cancer(NSCLC)cells to low linear energy transfer(LET)X-rays and high-LET carbon ions,clonogenic survival assays were performed to detect the radiosensitivity of NSCLC A549 cells following caspase-9b knowdown and ionizing radiations.The results revealed that high-LET carbon ions enhanced the alternative splicingmediated radiosensitivity within the exposure dose limits,but low-LET X-rays could increase the radiosensitivity in a dose-dependent manner.