AIM To prospectively evaluate the reproducibility of diaphragm thickness measurement by ultrasonography at the bedside by critical care physicians in patients on invasive mechanical ventilation.METHODS In a prospective observational study of 64 invasively ventilated patients,diaphragmatic thickness measurement was taken by 2 different observers at the same site.Three measurements were taken by each observer and averaged.The intraobserver and interobserver variability was assessed by estimation of intraclass correlation coefficient.The limits of agreement were plotted as the difference between two observations against the average of the two observations in Bland and Altman analysis.RESULTS The mean diaphragm thickness at the functional residual capacity was 2.29±0.4 mm and the lower limit of the normal,i.e.,the 5th percentile was 1.7 mm(95%CI:1.6-1.8).The intraclass correlation coefficient for intraobserver variability was 0.986(95%CI:0.979-0.991)with a P value of<0.001.The intraclass correlation coefficient for interobserver variability was 0.987(95%CI:0.949-0.997)with a P value of<0.001.In Bland and Altman analysis,both intraobserver and interobserver measurements showed high limits of agreement.CONCLUSION Our study demonstrates that the measurement of diaphragm thickness by ultrasound can be accurately performed by critical care physicians with high degree of reproducibility in patients on mechanical ventilation.