Denaturation of DNA in Ternary Mixed Solution of Water/Hydrophilic/Hydrophobic Organic Solvent

• 作者: Akira Kobayashi Kazuhiko Tsukagoshi Yuki Ito
• 来源期刊: 分析科学方法和仪器期刊(英文)
• 学科: 化学
• 关键词: DENATURATION DNA TERNARY MIXED SOLVENT HYDROPHOBIC Organic SOLVENT Absorption Behavior
• 年，卷（期）: fxkxffhyqqkyw,（2）
• 页码范围: 40-46
• 页数: 7页
• 分类号: O6

摘要

Denaturation was examined for the first time in a ternary mixed solution of water/hydrophilic/ hydrophobic organic solvent using λ-DNA and a plasmid as models. The absorbance of λ-DNA and the plasmid at 260 nm gradually increased for several days up to 1.68 and 1.38 times the initial values, respectively, in a water/acetonitrile/ethyl acetate (15:3:2, volume ratio) mixed solution, whereas there was little change in a water/acetonitrile (15:3, volume ratio) mixed solution. The plasmid treated with the ternary mixed solution was also examined with agarose gel electrophoresis. These experimental data indicated that λ-DNA changed from a double helix structure to a single helix structure and that the plasmid partially transformed to generate a denaturation bubble in the structure. The new idea of using the ternary mixed solution first enabled the interaction of the hydrophobic organic solvent (e.g., ethyl acetate) molecule with the double helical structure of DNA, leading to specific slow-proceeding denaturation.