Varicocele and DNA Fragmentation in Senegalese Infertile Males—A Study of 95 Cases

• 作者: Abdoulaye Séga Diallo Babacar Diao Jean Pascal Demba Diop Mama Sy Diallo Oumar Faye Rokhaya Ndiaye Diallo Yoro Diallo
• 来源期刊: 生殖科学(英文)
• 学科: 医学
• 关键词: VARICOCELE DNA FRAGMENTATION Male INFERTILITY TUNEL ASSAY
• 年，卷（期）: 2017,（3）
• 页码范围: 47-55
• 页数: 9页
• 分类号: R73

摘要

Varicocele is the first etiology of infertility in men worldwide and affects almost half of the men dealing with infertility in Senegal. Varicocele is associated with a high rate of sperm DNA fragmentation so this test is being more and more recommended especially when patients have to benefit from an ART (Assisted Reproductive Technology) procedure. The aim of our study was to assess if our patients with varicocele are concerned by higher DNA fragmentation rate in spermatozoon. Sperm samples were collected (95 cases of varicocele and 30 controls) and analyzed between January 2013 and June 2016 in a prospective study. Sperm DNA damage, characterized by the rate of DNA fragmentation was evaluated by TUNEL assay using fluorescence microscopy. There was a significant higher percentage of DNA fragmentation in patients with varicocele (p < 0.001), compared to controls. We plan to study a bigger cohort to assess this tendency and study the benefits of surgery in varicocele by evaluating DNA fragmentation after the procedure.