Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study

• 来源期刊: 中国昆虫科学（英文版）
• 关键词: Apis dorsata DGGE gut flora insect bacteria microbial diversity 16S rRNA gene
• 年，卷（期）: 2017,（1）
• 页码范围: 81-92
• 页数: 12页
• 语种: 英文
• DOI: 10.1111/1744-7917.12271

摘要

Bacterial communities are known to play important roles during the developmental stages of insects,but current knowledge of bacteria associated with the midgut of Apis dorsata,the giant Asian honeybee,is limited.Using polymerase chain reactiondenaturing gradient gel electrophoresis analysis (PCR-DGGE) and 16S rRNA sequencing,the aim of this study was to determine the dynamics of bacterial community structure across four A.dorsata life stages in different geographical locations.The results reveal that bacterial diversity increased as the bee progressed through larval stage to newly emerged worker and old worker.However,in the pupal stage,no bands identified as bacteria could be observed.Overall,2 bacterial phyla (Proteobacteria and Firrnicutes) and 4 classes (Alphaproteobacteria,Betaproteobacteria,Gammaproteobacteria,and Bacilli)were identified,but the frequency varied among the different stages and locations.The classes of Gammaproteobacteria and Bacilli dominated among larval,newly emerged worker and old worker developmental stages.