This paper presents a one-way data transmission method in order to ensure the safety of data transmission from mobile storage to secure PC.First,an optocoupler is used to achieve the one-way transmission of physical channel,so that data can only be transmitted from mobile storage to secure PC,while the opposite direction is no physical channel.Then,a safe and reliable software system is designed which contains one-way communication protocol,fast CRC check method and packet retransmission algorithm together to ensure the safety of data transmission.After that,to obtain the maximum transmission rate,the frequency of data bus(slwr)and the packet size(num)which effect on transmission rate are detailed analyzed.Experimental results show the proposed method is high-efficiency and safe.