The possibility of anaerobic digestion of high oil waste at high temperatures was explored, and the effects of fat concentration on biogas production via anaerobic digestion were analyzed by batch and continuous experiments successively. Besides, the response and recovery of the digestion system to the shock of high concentrations of oil were studied. The results showed that the anaerobic digestion of high oil waste could be carried out, and fat concentration had a significant effect on the anaerobic digestion. In the batch experiments, the process of anaerobic digestion could carry out only when fat concentration was 0-1.68% , and the average methane content was 46.42%;with the increase of fat concentration, both methane content and production rate decreased significantly, and the digestion period was extended obviously. When fat concentration exceeded 2.52%,the anaerobic digestion failed to start, and methane could not be detected in the produced biogas. In the continuous experiments, the system could withstand a certain concentration of fat (6g/L) , and anaerobic digestion process would be inhibited when it experienced the impact of a high oil load (26.7g/L) . Nevertheless, this process could recover quickly after the removal of the impact, and the system could withstand a higher fat concentration (13g/L) than before. Furthermore, the volume production rate of biogas was approximately 1 IV (L·d) , and methane content was about 55%.