MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2017,（6）
• 页码范围: 391-398
• 页数: 8页
• 语种: 英文

摘要

We described a novel single-cell RNA-seq technique called MR-seq (measure a single-cell transcriptome repeatedly),which permits statistically assessing the technical variation and identifying the differentially expressed genes between just two single cells by measuring each single cell twice.We demonstrated that MR-seq gave sensitivity and reproducibility similar to the standard single-cell RNA-seq and increased the positive predicate value.Application of MR-seq to early mouse embryos identified hundreds of candidate intra-embryonic heterogeneous genes among mouse 2-,4-and 8-cell stage embryos.MR-seq should be useful for detecting differentially expressed genes among a small number of cells.