Exploration of Activated Pathways for Improving Antifungal Agent FR901469 Productivity in Fungal Species No.11243 Using Comprehensive Pathway Model

• 作者: Hiroya Itoh Itaru Takeda Makoto Matsui Masayuki Machida Sachiyo Aburatani Takashi Shibata
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: BIOINFORMATICS Metabolic PATHWAY MODEL FUNGI Secondary METABOLITES
• 年，卷（期）: 2017,（7）
• 页码范围: 16-31
• 页数: 16页
• 分类号: R73

摘要

Secondary metabolites are important for various industrial applications. The production of secondary metabolites is often improved by the activation of substrate supply pathways for biosynthesis. However, many important pathways have remained unclear. In this study, we explored possible pathways related to substrate supply for the biosynthesis of the antifungal agent FR901469 which is a nonribosomal peptide and a fungal secondary metabolite. To clarify the unknown activated pathways, we utilized the Comprehensive Pathway Model (CPM) which was developed in our previous study. We verified that the overexpression of the hypothetical beta-alanine-aminotransferase (BAL-AT), which was included in the explored pathways, improved the FR901469 productivity. The genes encoding the BAL metabolic enzymes are considered to be important for improving the FR901469 productivity.