A Portable Evanescent Wave Optic Fiber Immunosensor for Sensitive and Rapid Detection of 2,4-D in Water Samples

• 作者: Dan Song Feng Long Jun Wu Rong Yang Wei Li
• 来源期刊: 地球科学和环境保护期刊(英文)
• 学科: 医学
• 关键词: EVANESCENT Wave OPTIC Fiber IMMUNOSENSOR 2 4-D Detection IMMUNOASSAY
• 年，卷（期）: 2017,（6）
• 页码范围: 42-45
• 页数: 4页
• 分类号: R73

摘要

A portable evanescent wave optic ?ber immunosensor was developed for detection of 2,4-dichlorophenoxyacetic acid(2,4-D) using a indirect competitive immunoassay. In this paper, hapten conjugates 2,4-D-OVA were immobilized with covalent binding methods. After pre-reacting, 2,4-D-antibody-Cy5.5 in sample specifically recognized the 2,4-D antigens binding sites on surface of the optical fiber probe. Under optimum conditions, 2,4-D could be detected in less than 18 min for each assay cycle. The regeneration of the optic ?ber surface allowed more than 200 times without losing performance. The limits of detection of 0.039 ug/L and the quantitative detection range of 0.47 - 81.02 ug/L were obtained when the concentration of 2,4-D was 1 mg/L. This immunosensor shows great potential in rapid simultaneous detection of 2,4-D in waters samples.