A new semi-quinonechalcone C-glycoside isocartormin along with cartormin and safflomin C were isolated from the water extract of Carthamus tinctorius L.The structure of isocartormin was determined by extensive analysis of HR-MS,1D-and 2D NMR data,and by comparison with those of cartormin reported previously by our group.Isocartormin was identified as a diastereoisomer of cartormin with a reverse configuration at C-18.