Based on the concept and connotation of water environment carrying capacity, taking Yunnan Province as a case, this paper built water environment carrying capacity evaluation system from the perspectives of water resources, water environment carrying capacity and socio-economic development, and applied the index evaluation model to analyze the trends of water environment carrying capacity in Yunnan from 2006 to 2014. The results showed that, during those years, the evaluation value of water environment carrying capacity ranged from 0.23 to 0.46 in Yunnan Province.The minimum value was 0.23 in 2013, the maximum value was 0.46 in 2010. From 2006 to 2014, the evaluation value of water environment carrying capacity was less than 0.5 in general, and the water environment in a fragile state on the whole.