The forest ecological system fulfills many important ecological functions. Researches on the analysis and valuation on forest ecosystem services are at the cutting edge of current ecology and ecological economics. Based on the calculation method of service value of forest ecosystem, 7 forest ecosystem services of Nanling National Nature Reserve were valued, totaling 703.48 million yuan/year. In terms of the contribution size of the service value of forest ecosystem, carbon fixation and oxygen release came as the greatest one, followed by conservation of soil, purification of the air, biodiversity conservation, conservation of water, accumulation of nutrients, and forest recreation. With its value of 208.46 million yuan, carbon fixation and oxygen release ranked first, accounting for 29.63% of the total value;conservation of soil of 24.67% service value took the second place;purification of the air came in third in the value rankings, representing 22.55% of the total value;the fourth one was biodiversity conservation, whose service value came up to 14.51%. Those 4 services made up 91.36% of the total value while other 3 services ranging from conservation of water, accumulation of nutrients to forest recreation were merely a small proportion. This is because the Nanling National Nature Reserve is essentially a large oxygen bar. The above-mentioned ecosystem valuation method contributes to the understanding of the overall value of the forest ecosystem as well as the implementation and advancement in the regional sustainable development strategy.