Effect of Competitive Antagonism of Caspase-9 Alternative Splicing Isoforms on the Radiosensitivity of NSCLC Cells

• 作者: Jin Xiaodong Kuang Yanbei Li Ping Li Qiang
• 来源期刊: 中国科学院近代物理研究所和兰州重离子研究装置年报:英文版
• 学科: 医学
• 关键词: NSCLC lung mutual
• 年，卷（期）: 2017,（1）
• 页码范围: 140-141
• 页数: 2页
• 分类号: R73

摘要

Alternate RNA processing of caspase-9 generates the splice variants caspase-9a(C9a)and caspase-9b(C9b)[1].C9b lacks a domain present in C9a,revealing a tumorigenic function that drives the phenotype of non–small cell lung cancer(NSCLC).By comparing NSCLC cells with wild and null p53 status,the results from competitive RT-PCR assay displayed that in the p53 wild-type cell line A549,caspase-9b siRNA and X-irradiation were administered to significantly increase the ratio of C9a/C9b;in the p53-null NCI-H1299 cell line,as long as caspase-9b siRNA was performed,regardless of whether irradiation increased the ratio of C9a/C9b,there was no significant difference between the two groups(Fig.1).It suggests that p53 plays an important role in CASP-9a/CASP-9b mutual antagonism and subsequent radiosensitivity induced by X-ray.