Protein-membrane interactions investigated with surface-induced fluorescence attenuation

• 来源期刊: 中国物理B（英文版）
• 关键词: single molecule surfaceinduced fluorescence attenuation membrane-protein interactions molecular mechanism
• 年，卷（期）: 2017,（12）
• 页码范围: 62-67
• 页数: 6页
• 语种: 英文
• DOI: 10.1088/1674-1056/26/12/128708

摘要

Research on protein-membrane interactions has been undeveloped due to the lack of proper techniques to detect the position of proteins at membranes because membranes are usually only about 4-nm thick.We have recently developed a new method named surface-induced fluorescence attenuation (SIFA) to track both vertical and lateral kinetics of a single labelling dye in supported lipid bilayers.It takes advantage of strong interaction between a light-emitting dye and a partially reflecting surface.By applying the technique to membrane proteins being fluorescently labelled at different residues,here we show that SIFA can measure not only the insertion depth of a dye inside a lipid bilayer,but also the position of a dye in solution near the surface.SIFA can therefore be used to study membrane proteins of various types.