Chemical synthesis of histone H2A with methylation at Gln104

• 来源期刊: 中国科学:化学（英文版）
• 年，卷（期）: 2017,（5）
• 页码范围: 621-627
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s11426-016-0386-4

摘要

Histone H2A methylation at Gln 104 (H2AQ 104Me) is a new type of histone post-translational modification (PTM) discovered recently.This modification has been found to have significant influence on gene transcription.However,the structural and functional consequence of glutamine methylation on nucleosome remains to be further elucidated.Obtaining of histones with site-specific methylation at glutamine residues might facilitate the studies towards a better understanding of this new PTM.In the present work,total chemical synthesis of H2AQ104Me was carried out through use of the hydrazide-based native chemical ligation.Synthetic histone H2AQ104Me could be successfully incorporated into nucleosomes in vitro and showed a negative influence on the nucleosome stability.