MiR-200c suppresses the migration of retinoblastoma cells by reversing epithelial mesenchymal transition

• 作者: Ling Gao Xiao-Lei Shao Yao Chen
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 年，卷（期）: 2017,（8）
• 页码范围: 1195-1202
• 页数: 8页
• 分类号: R77

摘要

AIM:To analyze the relationship between clinical features and epithelial mesenchymal transition(EMT) in retinoblastoma(RB),further to investigate whether miR-200c regulates the EMT and migration of RB cells.METHODS: Expression of EMT-related markers and tumorrelated factors were detected by immuno-histochemistry analysis in RB tissue from 29 cases. Correlations between their expression and clinical characteristics were analyzed. The regulation effects of miR-200c on EMT-related markers,tumor-related factors were observed in m RNA level and protein level by real-time polymerase chain reaction(PCR) and Western blot,respectively,in Y79 and Weri-rb1 cells. Its effects on migration force of these RB cell lines were also detected with Transwell test.RESULTS: Lower expression of E-cadherin was present in the cases with malignant prognosis. Mi R-200c promoted the expression of E-cadherin and decreased the expression of Vimentin and N-cadherin in Y79 and Weri-rb1 cells. Migration force of RB cells could be inhibited by miR-200c.CONCLUSION: EMT might be associated with bad prognosis in RB. Mi R-200c suppresses the migration of retinoblastomatous cells by reverse EMT.