A peptide-based near-infrared fluorescence probe for dynamic monitoring senile plaques in Alzheimer's disease mouse model

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2017,（23）
• 页码范围: 1593-1601
• 页数: 9页
• 语种: 英文

摘要

In vivo monitoring neuropathological changes in Alzheimer's disease (AD) animal model is critical for drug development.Here,by integrating blood-brain barrier penetrable peptide,we have developed a peptide probe which based on angiopep-2.Angiopep-based probe exhibited high binding affinity to Aβ aggregates and labeled senile plaques in vivo.Remarkably,the in vivo near-infrared imaging data revealed that fluorescence signals of this probe were nearly 3-fold higher in the brains of 16-monthold APP/PS1 transgenic mice compared to C57 mice and exhibited linear correlation with the senile plaques load process in 4-,8-,16-month-old APP/PS1 transgenic mice.Moreover,senile plaques load was detected in vivo as early as 4 months of age that even at the very beginning of plaques developed in APP/PS1 transgenic mice.Taken together,this novel peptide-based probe achieved dynamic monitoring senile plaques in APP/PS1 transgenic mice and have been ready to use in drug development in AD mouse model.