A Simplified Approach for Olive (<i>Olea europaea</i>L.) Genotyping and Cultivars Traceability

• 作者: A. Manca Diego Breviario L. Braglia P. Hatzopoulos S. Gianì
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: OLIVE GENOTYPING TUBULIN Based Polymorphism SSRS Molecular Probes
• 年，卷（期）: mgzwxqkyw_2017,（13）
• 页码范围: 3475-3489
• 页数: 15页
• 分类号: R73

摘要

The Tubulin Based Polymorphism (TBP) method was used to genotype olive cultivars of different origin and to produce short-size cultivar-specific molecular probes. Both the first and the second intron of the members of the olive β-tubulin gene family were exploited as sources of DNA polymorphism. Compared with the data obtained with the use of a set of 11 SSR markers selected from an Olea europea L. database, TBP is shown to provide similar, if not better, information about the polymorphic content of the olive genomes, releasing, at the same time, a simple and discriminatory DNA barcode specific for any of the analyzed cultivars. Such a barcode is the source for the preparation of variety specific molecular probes.