Nigrospora sphaerica Products from the Flowering Dogwood Exhibit Antitumorigenic Effects via downregulation of the Translational Regulator,Ribosomal S6 Protein

• 作者: Margaret Mmbaga Quincy A.Quick SProtein Asha Maheshwari
• 来源期刊: 抗癌研究
• 学科: 医学
• 关键词: natural product fungi secondary METABOLITE intracellular signaling cancer
• 年，卷（期）: kayj_2018,（3）
• 页码范围: 8-15
• 页数: 8页
• 分类号: R73

摘要

The utility of diverse species of endophytic fungi as a viable source for drug agents with clinical applicability for the treatment of human diseases continues to expand.In this study,we examined secondary metabolites of Nigrospora sphaerica isolated from the flowering dogwood,Cornus florida L.,for their anticancer properties on lung cancer and glioblastoma.Molecular identification of N.sphaerica was determined using internal transcribed spacer rDNA sequence.The expression of translational pathway proteins was examined after exposure to various crude extract concentrations(2μg/ml,4μg/ml,and 8μg/ml)using immunoblotting procedures,while tumor cell migration analysis was performed using Boyden chamber assays.Crude N.sphaerica extracts exhibited antiproliferative and antimigratory effects on solid tumors as determined by cell proliferation and cell migration assays,respectively.The antitumorigenic effects of N.sphaerica were as a consequence of negatively regulating the PI3K/Akt/mTOR translational control signaling pathway,a canonical mechanistic axis that contributes to the maintenance and progression of several human cancers.To the best of our knowledge,this is the first evidence that demonstrates N.sphaerica from C.florida inhibits tumor cell migration and thus disease recurrence a major factor in the therapeutic resistance of cancers to chemotherapeutic agents.