Combinatorial Enzyme Approach to Produce Oligosaccharides of Diverse Structures for Functional Screen

• 作者: Dominic W. S. Wong Doris Feng, Sarah Batt William Orts
• 来源期刊: 酶研究进展(英文)
• 学科: 医学
• 关键词: Combinatorial ENZYME POLYGALACTURONASE Pectate LYASE PECTIN OLIGOSACCHARIDES
• 年，卷（期）: myjjzyw,（2）
• 页码范围: 11-20
• 页数: 10页
• 分类号: R73

摘要

Combinatorial chemistry has been a focus of research activity in modern drug discovery and biotechnology. It is a concept by which a vast library of molecular diversity is synthesized and screened for target properties. This report is to illustrate the application of enzyme technology using the concept of combinatorial chemistry as a novel approach for the bioconversion of plant fibers. Citrus pectin was subjected to combinatorial enzyme digestion to create libraries of pectic oligosaccharides with diverse structural variants. Repeated cycles of fractionation and screening resulted in the isolation and identification of an active oligoGalA species with antimicrobial activity.