A Simple Procedure for Extracting DNA from Coagulated Blood Samples for DNA Banking

• 作者: Cheong-Nang Kuok Grace Meng Jia Xu Khyati Chandratre Lu-Han Jiang San-Ming Wang Yuan-Qiang Lu
• 来源期刊: 脑血管病电子杂志(英文)
• 学科: 医学
• 关键词: DNA extraction DNA BANKING Coagulated BLOOD
• 年，卷（期）: 2018,（1）
• 页码范围: 12-17
• 页数: 6页
• 分类号: R

摘要

Background: DNA banking is increasingly applied to collect genetic materials for biomedical studies. Traditionally, the source of DNA banking is from the anti-coagulated blood samples as it is easy to isolate cells from the samples for DNA extraction. However, substantial portion of clinical blood samples is coagulated. Because of the difficulty to release blood cells from the clots for DNA extraction, coagulated blood samples are not used for DNA banking. Methods: In this study, we developed a simple procedure for high-throughput DNA extraction from coagulated blood samples for DNA banking. Results: We showed that the extracted DNA is the same as the one extracted from anti-coagulated blood samples as reflected by the size distribution and genetic variants across all coding genes. Conclusions: We developed a simply procedure for high-throughput DNA extraction from coagulated blood cells. Validation results show that the DNA from coagulated blood cells is the same as the DNA from the anti-coagulated blood cells.