Saccharification of Sugarcane Bagasse Using an Enzymatic Extract Produced by Aspergillus fumigatus

• 作者: Daniel Pasquini Kenia F.R.Lamounier Milla A.Baffi Patrisia O.Rodrigues
• 来源期刊: 可再生材料杂志(英文)
• 学科: 化学
• 关键词: A.fumigatus ENZYMATIC HYDROLYSIS SUGARCANE BAGASSE alkaline pretreatment solid state fermentation
• 年，卷（期）: kzsclzzyw,（2）
• 页码范围: 169-175
• 页数: 7页
• 分类号: O61

摘要

This study investigates the efficiency of a crude enzymatic extract produced by Aspergillus fumigatus SCBM6 by solid state fermentation(SSF)in the hydrolysis of alkali pretreated sugarcane bagasse(PTB).After SSF using in natura sugarcane bagasse(SCB),the enzymatic extract presented 21.33 U.g^-1 of β-glucosidase and 544.46 U.g^-1 of xylanase.The alkaline pretreatment with sodium hydroxide 2%NaOH(w/v)removed 43% of the lignin from PTB and the cellulosic fraction increased to 75%.The hydrolysis was optimized as a function of time,temperature,and concentration of PTB.After hydrolysis,the maximum yield(30.05%)of total released reducing sugars(TRS)was obtained under the following conditions:24 h,55℃,2% of PTB and 3 U.g^-1 of β-glucosidase(CBU).Furthermore,an approximate TRS value(26.4%)was also obtained after saccharification carried out during 6 h,55℃,4% of PTB and 1 CBU.These results indicate that hydrolysis can be performed in a short incubation period and with low enzymatic load for reasonable TRS release.