In this study, the detection method of tea saponin has been studied firstly. Determining the maximum absorption wavelength is 540 nm. Standard curve equation is y = 0.0015x - 0.0885. The correlation coefficient r = 0.9983 (p < 0.01). The relative standard deviation is 1.13%. Reclaimable rate of adding standard sample is 89.5% - 97.7%. Comparing vanille-sulfuric acid of gravimetric determination, the maximum deviation is 3.27%, indicating that vanille-sulfuric acid method is worth of quantitative analysis of tea saponin.