Analysis of short fruiting branch gene and Marker-assisted selection with SNP linked to its trait in upland cotton
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摘要:
Background:With the rapid development of genomics,many functional genes have been targeted.Molecular marker assisted selection can accelerate the breeding process by linking selection to functional genes.Methods:In a study of upland cotton (Gossypium hirsutum L.),the F2 segregated population was constructed by crossing X1570 (short branches) with Ekangmian-13 (long branches) to identify the short fruiting branch gene and marker-assisted selection with SNP(Single Nucleotide Polymorphisms,SNP) linked to its trait.Result:The result demonstrated that linked SSR marker BNL3232 was screened by BSA(Bulked segregant analysis,BSA) method;one SNP locus was found,which was totally separated from the fruiting branches trait in upland cotton.Conclusion:It was verified that this SNP marker could be used for molecular assisted selection of cotton architecture.