Morphological and biochemical effects of weekend alcohol consumption in rats:Role of concentration and gender

• 作者: ángel Morales-González Eduardo Madrigal-Bujaidar Eduardo Osiris Madrigal-Santillán Germán Chamorro-Cevallos Iselaálvarez-González JoséA Morales-González Laura Ligía González-López Liliana Anguiano-Robledo María de Lourdes Sernas-Morales Nancy Vargas-Mendoza Tomás Alejandro Fregoso-Aguilar
• 来源期刊: 世界肝病学杂志：英文版（电子版）
• 学科: 医学
• 关键词: WEEKEND ALCOHOL CONSUMPTION Liver morphology TRANSAMINASES Damage
• 年，卷（期）: 2018,（2）
• 页码范围: 297-307
• 页数: 11页
• 分类号: R

摘要

AIM To examine the association between weekend alcohol consumption and the biochemical and histological alterations at two different concentrations of alcohol in both genders in rats.METHODS Wistar rats weighing 170-200 g were divided into groups as follows:(1)Control groups;and(2)weekend alcohol-consumption group:2 d/weekly per 12 wk,at two different concentrations:(1)Group of males or females with a consumption of a solution of alcohol at 40%;and(2)group of males or females with a consumption of a solution of alcohol at 5%.At the end of the experiment,serum and liver samples were obtained.The following enzymes and metabolites were determined in serum:Alanine Aminotransferase(ALT),Aspartate Aminotransferase(AST),Lactate Dehydrogenase,and Gamma-Glutamyltransferase,and glucose,triglycerides,cholesterol,bilirubin,and albumin.Liver samples from each group were employed to analyze morphological abnormalities by light microscopy.RESULTS In all of the weekend alcohol-consumption groups,AST activity presented a significant,10-fold rise.Regarding ALT activity,the groups with weekend alcohol consumption presented a significant increase that was six times greater.Bilirubin levels increased significantly in both groups of females.We observed a significant increase in the parameters of fatty change and inflammation due to weekend alcohol consumption.Only the group of females that consumed alcohol at 40%presented slight hepatocellular disorganization CONCLUSION The results obtained herein provide solid evidence that weekend alcohol consumption gives rise to liver damage,demonstrated by biochemical and histological alterations,first manifested acutely,and prolonged weekend alcohol consumption can cause greater,irreversible damage.