Separation and simultaneous quantitation of PGF2α and its epimer 8-iso-PGF2α using modifier-assisted differential mobility spectrometry tandem mass spectrometry

• 来源期刊: 药学学报（英文版）
• 关键词: Differential mobility spectrometry Mass spectrometry Epimer PGF2α 8-iso-PGF2α
• 年，卷（期）: 2018,（2）
• 页码范围: 228-234
• 页数: 7页
• 语种: 英文

摘要

Because many therapeutic agents are contaminated by epimeric impurities or form epimers as a result of metabolism,analytical tools capable of determining epimers are increasingly in demand.This article is a proof-of-principle report of a novel DMS-MS/MS method to separate and simultaneously quantify epimers,taking PGF2α and its 8-epimer,8-iso-PGF2α,as an example.Good accuracy and precision were achieved in the range of 10-500 ng/mL with a run time of only 1.5 min.Isopropanol as organic modifier facilitated a good combination of sensitivity and separation.The method is the first example of the quantitation of epimers without chromatographic separation.