Molecular Variability and Genetic Structure of IYMV in Burkina Faso

• 作者: Bouma James Neya Christophe Brugidou Drissa Sérémé Eric Lacombe Florence Vignols Martine Bantgratz Moustapha Koala Nicolas Barro Oumar Traoré
• 来源期刊: 美国植物学期刊(英文)
• 学科: 医学
• 关键词: Imperata YELLOW MOTTLE virus (IYMV) COAT Protein (CP) Genetic Var-iability PHYLOGENETIC Analysis
• 年，卷（期）: 2018,（2）
• 页码范围: 311-324
• 页数: 14页
• 分类号: R73

摘要

Imperata yellow mottle virus (IYMV, Sobemovirus) was first described in 2008 in the south-western region of Burkina Faso (West Africa). The genetic diversity of IYMV was not documented up to day. In this study, the variability of CP of IYMV was evaluated through the molecular characterization of 38 isolates collected in the western part of Burkina Faso. Comparison of sequences of these new isolates and one IYMV sequence available in GenBank revealed that the average nucleotide diversity was low. The ratio of non-synonymous over synonymous nucleotide substitutions per site was low, indicating a CP diversification under strong purifying selection. Despite of the low nucleotide diversity, phylogenetic analyses revealed segregation of IYMV isolates into six major clades. There was no correlation of phylogenetic grouping of isolates based on geographical location. This is the first study of the genetic diversity of IYMV.