SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress

• 来源期刊: 分子细胞生物学报（英文版）
• 年，卷（期）: 2018,（5）
• 页码范围: 460-474
• 页数: 15页
• 语种: 英文
• DOI: 10.1093/jmcb/mjy047

摘要

Human flap endonuclease 1 (FEN1) is a structure-specific,multi-functional endonuclease essential for DNA replication and repair.We and others have shown that during DNA replication,FEN1 processes Okazaki fragments via its interaction with the proliferating cell nuclear antigen (PCNA).Alternatively,in response to DNA damage,FEN1 interacts with the PCNA-like Radg-Rad1-Hus1 complex instead of PCNA to engage in DNA repair activities,such as homology-directed repair of stalled DNA replication forks.However,it is unclear how FEN1 is able to switch between these interactions and its roles in DNA replication and DNA repair.Here,we report that FEN1 undergoes SUMOylation by SUMO-1 in response to DNA replication fork-stalling agents,such as UV irradiation,hydroxyurea,and mitomycin C.This DNA damage-induced SUMO-1 modification promotes the interaction of FEN1 with the Rad9-Rad1-Hus1 complex.Furthermore,we found that FEN1 mutations that prevent its SUMO-1 modification also impair its ability to interact with HUS1 and to rescue stalled replication forks.These impairments lead to the accumulation of DNA damage and heightened sensitivity to fork-stalling agents.Altogether,our findings suggest an important role of the SUMO-1 modification of FEN1 in regulating its roles in DNA replication and repair.