LRIF1 interacts with HP1α to coordinate accurate chromosome segregation during mitosis

• 来源期刊: 分子细胞生物学报（英文版）
• 年，卷（期）: 2018,（6）
• 页码范围: 527-538
• 页数: 12页
• 语种: 英文
• DOI: 10.1093/jmcb/mjy040

摘要

Heterochromatin protein 1α (HP1α) regulates chromatin specification and plasticity during cell fate decision.Different structural determinants account for HP1α localization and function during cell division cycle.Our earlier study showed that centromeric localization of HP1α depends on the epigenetic mark H3K9me3 in interphase,while its centromeric location in mitosis relies on uncharacterized PXVXL-containing factors.Here,we identified a PXVXL-containing protein,ligand-dependent nuclear receptorinteracting factor 1 (LRIF1),which recruits HP1α to the centromere of mitotic chromosomes and its interaction with HP1α is essential for accurate chromosome segregation during mitosis.LRIF1 interacts directly with HP1α chromoshadow domain via an evolutionarily conserved PXVXL motif within its C-terminus.Importantly,the LRIF1-HP1α interaction is critical for Aurora B activity in the inner centromere.Mutation of PXVXL motif of LRIF1 leads to defects in HP1α centromere targeting and aberrant chromosome segregation.These findings reveal a previously unrecognized direct link between LRIF1 and HP1α in centromere plasticity control and illustrate the critical role of LRIF1-HP1α interaction in orchestrating accurate cell division.