Subcutaneous Model for the Study of Dengue Virus Infection in Immune Competent Mice

• 作者: É Alexandre Vieira Machado Beatriz Senra Santos Bruno Galvã Erna Geessien Kroon Gustavo Cardoso de Oliveira Ketyllen Reis Andrade Marcele Neves Rocha Marco Antô Natalia Lima Pessoa Natalia Lucinda nio Campos o Filho Pedro Augusto Alves rica Alessandra Rocha Alves Thais Souza Silva
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: SUBCUTANEOUS Murine MODEL DENGUE Disease IMMUNE COMPETENT Mouse Cytokines INNATE IMMUNE Response
• 年，卷（期）: 2018,（5）
• 页码范围: 97-110
• 页数: 14页
• 分类号: R73

摘要

Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent Dengue virus (DENV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.