Elaboration of Materials with Functionality Gradients by Assembly of Chitosan-Collagen Microspheres Produced by Microfluidics

• 作者: David Azria Emmanuel Belamie Frédéric Gobeaux Marie Morille Raluca Guermache Sébastien Blanquer Sophie Raisin
• 来源期刊: 可再生材料杂志(英文)
• 学科: 化学
• 关键词: Hybrid MICROSPHERES BIOMATERIALS chitosan collagen FUNCTIONALITY gradient nerve regeneration MICROFLUIDICS
• 年，卷（期）: kzsclzzyw_2018,（3）
• 页码范围: 314-324
• 页数: 11页
• 分类号: O63

摘要

Biopolymers extracted from renewable resources like chitosan and collagen exhibit interesting properties for the elaboration of materials designed for tissue engineering applications,among which are their hydrophilicity,biocompatibility and biodegradability.In many cases,functional recovery of an injured tissue or organ requires oriented cell outgrowth,which is particularly critical for nerve regeneration.Therefore,there is a growing interest for the elaboration of materials exhibiting functionalization gradients able to guide cells.Here,we explore an original way of elaborating such gradients by assembling particles from a library of functionalized microspheres.We propose a simple process to prepare chitosan-collagen hybrid microspheres by micro-and milli-fluidics,with adaptable dimensions and narrow size distributions.The adhesion and survival rate of PC12 cells on hybrid microspheres were compared to those on pure chitosan ones.Finally,functionalized microspheres were assembled into membranes exhibiting a functionalization gradient.