Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution

• 来源期刊: 光：科学与应用（英文版）
• 关键词: cranial window dendritic spines optical clearing synaptic resolution two-photon imaging
• 年，卷（期）: 2018,（1）
• 页码范围: 133-141
• 页数: 9页
• 语种: 英文
• DOI: 10.1038/lsa.2017.153

摘要

Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena.Here,we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution.Combined with two-photon microscopy,this technique allowed us to repeatedly image neurons,microglia and microvasculature of mice.We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation.Given its easy handling and safety,this method holds great promise for application in neuroscience research.