Heparin Sodium Was Prepared from Pig Intestinal Mucosa by Dialysis and Spray Drying

• 作者: Caixia Yang Chonghuan Bao Jun Niu Qian Mi Shunshun Shu Zhaoyang Wang
• 来源期刊: 生物科学与医学(英文)
• 学科: 医学
• 关键词: HEPARIN Sodium DIALYSIS and Spray DRYING OVEN DRYING Method The Production Process
• 年，卷（期）: 2018,（10）
• 页码范围: 5-11
• 页数: 7页
• 分类号: R73

摘要

A method to extract crude heparin sodium from pig intestinal mucosa by dialysis and spray drying was established. The pig intestinal mucosa was treated in the following steps: enzymolysis, resin exchange adsorption-washing, elution, pressure filtration, dialysis, spray drying. Activity of the product was measured using a heparin anti-IIa factor assay kit. The yield of crude heparin obtained by this method was 2.79% higher than that of oven drying method;the production of 1 kg crude heparin sodium saved 43.4 pigs small intestine. The activity was 98.48 ± 2.49 IU/mg (n = 5), 15.18 IU/mg higher than that obtained by oven drying method. The product is pale white powder, attractive color and easy to dissolve.