In this paper, a total of 723 traditional villages in Hunan were selected for research. With the aid of spatial analysis of GIS, the number equilibrium and spatial autocorrelation of the traditional villages were analyzed quantitatively, watershed distribution characteristics and ethnic distribution characteristics of the traditional villages were analyzed both qualitatively and quantitatively, and the influence factors of distribution of the traditional village were explored in terms of three aspects of agricultural production and population migration, history and culture, and economic activities.