Super-resolution Imaging of Chromatin Remodeling Induced by Ionizing Irradiation

• 作者: Du Guanghua Liu Wenjing Shen Cheng Wu Ruqun
• 来源期刊: 中国科学院近代物理研究所和兰州重离子研究装置年报:英文版
• 学科: 政治法律
• 关键词: DAMAGE DSB BREAK
• 年，卷（期）: 2018,（1）
• 页码范围: 179-180
• 页数: 2页
• 分类号: D99

摘要

Radiation-induced DNA damages include base deletion,single-strand break and double-strand break(DSB).Among them,DSB is the most harmful,which could leads to gene mutation,cell apoptosis or carcinogenesis[1].Histone H2AX was phosphorylated(-H2AX)at Ser-139 rapidly after DSB damage.Quantitative analysis of-H2AX focus by fluorescence microscopy has become the popular method for DSB detection that each break has been found to correspond to one-H2AX focus.-H2AX plays an important role in damage signal cascading that the modification of chromatin improves DNA accessibility and leads to the recruitment and accumulation of specific DNA damage response(DDR)proteins at the DNA end[2,3].