Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

• 来源期刊: 中国物理B（英文版）
• 关键词: biophysics G-quadruplexes single-molecule studies DNA structures
• 年，卷（期）: 2018,（6）
• 页码范围: 541-551
• 页数: 11页
• 语种: 英文
• DOI: 10.1088/1674-1056/27/6/068701

摘要

Human telomeric G-quadruplex plays a crucial role in regulating the genome stability.Despite extensive studies on structures and kinetics of monomeric G-quadruplex,the interaction between G-quadruplexes is still in debate.In this work,we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K+ buffer.The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed.The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes.Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.